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This trend was also found at the 1- and 2-hour time points fig. S7, C to E. Together, these experiments demonstrate that GtaC shuttling is required for proper control of developmental gene expression and development progression. S8, A and B. Thus, in wild-type cells, pulses of cAMP are more effective as signals in promot- ing development than continuous stimuli because the former allow repeated nuclear entry of GtaC, whereas the latter keep GtaC in the cytosol.
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